Target of this antibody:
Alternative names of antibody target:
IL-18, IL18, IL 18, Interluekin-18, Interluekin 18, interluekin
IL-18 (IFN-gamma-inducing factor, IGIF) is a novel proinflammatory cytokine that aμgments natural killer (NK) activity in spleen cells. It also has other biological properties such as activation of NF-kB, stimulation of Fas ligand expression, induction of CC and CXC chemokines. IL-18 increases production of competent HIV. IL-18 is related to the IL-1 family. One of the IL-18 receptor chains is the IL-1 receptor-related protein, a member of the IL-1R family. Like IL-1β, IL-18 is first synthesized as a leaderless precursor requiring the IL-1β converting enzyme (ICE) for cleavage into an active molecule. IL-18 may play a role in tissue injury in inflammatory reactions.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of mouse IL-18
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-mouse IL-18 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Neutralization
Western blot analysis (0.5-4 µg/ml), immunoprecipitation (2-5 µg/ml), and neutralization (2-5 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.