Target of this antibody:
Alternative names of antibody target:
GRO alpha, GRO, chemokine (C-X-C), KC
KC, a homolog of human and hamster gro/MGSA, is a 72-amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of neutrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1-gamma stimulation. IFN-g blocks LPS-induced expression of KC.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 78 of mouse KC
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 5212BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.