Target of this antibody:
Alternative names of antibody target:
LEP; OB; OBS; Obese Protein, Leptin
Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acide residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.
Clonality of antibody:
This antibody reacts with:
E. coli-expressed recombinant human leptin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit anti-human Leptin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Leptin (Cat.# 4366-1) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.