anti-3-Nitrotyrosine Antibody (39B6)

Catalog number
5412-100
Name
anti-3-Nitrotyrosine Antibody (39B6)
Size
3-Nitrotyrosine Antibody (39B6)
Price
316.00 EUR
Supplier

Details

Target of this antibody:
3-Nitrotyrosine
Alternative names of antibody target:
3-NTyr
Background information:
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG2a
Clone:
Clone 39B6
This antibody reacts with:
All species
Immunogene sequence:
3-(4-Hydroxy-3-nitrophenylacetamido) propionic acid-BSA conjμgate.
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) in PBS containing 0.09% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western Blot, ELISA (with antigen coated plates), Immunohistochemistry (frozen sections, paraffin sections), Immunoprecipitation
Recommended usage:
Western Blot (1:5000), ELISA (with antigen coated plates), Immunohistochemistry (frozen sections, paraffin sections), Immunoprecipitation
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps