Target of this antibody:
Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant Human IL-12 p40 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids fragment (23-328).
Clonality of antibody:
This antibody reacts with:
E. coli expressed recombinant human IL-12
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-human IL-12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). It detects mainly the 40 kDa of IL-12p40 isoform, and in a lesser extent the 35 kDa of IL-12p35 isoform. Recombinant human IL-12 (Cat.#:4732-10) can be used as a positive control. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.