TNF-alpha, human recombinant

Catalog number
1050-10
Name
TNF-alpha, human recombinant
Size
10 μg
Price
240.00 EUR
Supplier

Details

Synonyms
TNF, Tumor necrosis factor- alpha
Alternates names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Taglines
A potent cytokine exerting cytotoxic effects on a wide range of cells
Biomolecule/Target
NA
NCBI Gene ID #.
7124
NCBI Gene Symbol
TNFA
Gene Source
Human
Accession #
P01375
Recombinant
Yes
Source
E. coli.
Purity
≥98%
Endotoxin Level
N/A
Activity (Specifications/test method)
N/A
Biological activity
ED50 <0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
Results
N/A
Molecular Weight
17.4 kDa
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized in 50 mM Tris, 150 mM NaCl pH 8.5
Reconstitution Instructions
Reconstitute in H₂O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Description
The TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.