TNF-alpha, rat recombinant

Catalog number
1052-1000
Name
TNF-alpha, rat recombinant
Size
1 mg
Price
3000.00 EUR
Supplier

Details

Synonyms
TNF, Tumor necrosis factor- alpha
Alternates names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Taglines
A potent cytokine exerting cytotoxic effects on a wide range of cells
Biomolecule/Target
TNF-alpha
NCBI Gene ID #.
24835
NCBI Gene Symbol
TNFA
Gene Source
Rat
Accession #
P16599.1
Recombinant
Yes
Source
E. coli.
Purity
≥98%
Activity (Specifications/test method)
N/A
Biological activity
The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.05 ng/ml, corresponding to a specific activity of> 2 x 10⁷ units/mg.
Results
N/A
Molecular Weight
17.0 kDa
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered and lyophilized from 1X PBS
Reconstitution Instructions
We recommend a quick spin followed by reconstitution in H₂O to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Description
The TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.