anti-DNA Methyltransferase 1 (Clone 60B1220.1) Antibody

Catalog number
anti-DNA Methyltransferase 1 (Clone 60B1220.1) Antibody
DNA Methyltransferase 1 (Clone 60B1220.1) Antibody
295.00 EUR


Target of this antibody:
Alternative names of antibody target:
Background information:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. DNMT1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites sμggesting a link between DNA methylation, histone deacetylase, and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Antibody host:
Clonality of antibody:
Mouse IgG1κ
This antibody reacts with:
Human, mouse, zebrafish
Immunogene sequence:
Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human DNMT1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Purification method:
Antigen affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation
Recommended usage:
Western blot: 2-4 µg/ml. IHC (parffin-embedded sections), ChIP, and IP: 1-2 µg/ml. However, the optimal conditions should be determined individually.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation