anti-DNA Methyltransferase 3a (Clone 64B1446) Antibody

Catalog number
6115-50
Name
anti-DNA Methyltransferase 3a (Clone 64B1446) Antibody
Size
DNA Methyltransferase 3a (Clone 64B1446) Antibody
Price
295.00 EUR
Supplier

Details

Target of this antibody:
DNMT3A
Alternative names of antibody target:
DNMT3, DNMT3A
Background information:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1κ
Clone:
64B1446
This antibody reacts with:
Human, mouse
Immunogene sequence:
Recombinant mouse Dnmt3a expressed in bacteria. The epitope was found to be located near the C-terminus (amino acids 705-908).
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Purification method:
Protein G purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blot: 2 µg/ml, ICC/IF: 5 µg/ml, ChIP/IHC (paraffin embedded sections). However, the optimal conditions should be determined individually.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps