Target of this antibody:
Alternative names of antibody target:
AD-019, CGI-70, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase, UCH37, Ubiquitin thioesterase L5, UCH37, UCH-L5, (Ubiquitin C-terminal hydrolase L5
Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all possess a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL5 is the only ubiquitin carboxy-terminal hydrolase (UCH)-family protease that is associated with mammalian proteasomes. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. It is responsible for the ubiquitin isopeptidase activity in the PA700 (19S) proteasome regulatory complex.
Clonality of antibody:
This antibody reacts with:
Recombinant full length protein
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
50 µg of antibody in PBS containing 10% glycerol
Purified from egg yolk
This antibody has been succesfully tested for use in following methods:
Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.