Target of this antibody:
Alternative names of antibody target:
Deubiquitinating enzyme 15, KIAA0529, MGC131982, MGC74854, Ubiquitin carboxyl-terminal hydrolase 15, Ubiquitin-specific-processing protease 15, Ubiquitin thioesterase 15, Unph-2, Unph4, UNPH4, Ubiquitin-specific-processing protease 15
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP15 (ubiquitin specific peptidase 15), also known as UNPH4, is a member of the peptidase C19 family of proteins. Expressed in kidney, liver, placenta, ovary, lung, thymus, heart and skeletal muscle, USP15 localizes to the cytoplasm and the nucleus, contains one DUSP domain and functions as a deubiquitinating enzyme that cleaves ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. Via its DUSP domain, USP15 plays a role in the regulation of the COP9 signalosome (CSN) complex. Three isoforms exist for USP15 due to alternative splicing events.
Clonality of antibody:
This antibody reacts with:
Recombinant full length protein
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
50 µg of antibody in PBS containing 10% glycerol
Purified from egg yolk
This antibody has been succesfully tested for use in following methods:
Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.