anti-SIRT1 Antibody

SIRT1

SIRT1, SIR2L1

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. This SIRT family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

C terminal peptide of human SIRT1

N/A

Liquid

100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3

Affinity purified

Western blot, Immunohistochemistry

Western blot: 1:500 – 1:2000 dilution, IHC: 1:50 - 1:200 dilution. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps