Target of this antibody:
Alternative names of antibody target:
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Fusion protein of human USP25
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
This antibody has been succesfully tested for use in following methods:
Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.