Target of this antibody:
Alternative names of antibody target:
CBX5, HP1, HP1A
Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; However, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino terminal chromodomain, followed by a variable hinge region and a conserved carboxyterminal chromoshadow domain. The chromodomain facilitates binding to histone H3 trimethylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatinassembly factor1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of posttranslational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Fusion protein of human CBX5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.