Target of this antibody:
Alternative names of antibody target:
In the DNA of higher eukaryotes, hydrolytic deamination of 5methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon nitrogen bond between the sugar phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitinlike proteins SUMO1 and SUMO2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Fusion protein of human TDG
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blot: 1:500 – 1:1000.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.