Active Sonic Hedgehog (SHH), human recombinant

Catalog number
4010-100
Name
Active Sonic Hedgehog (SHH), human recombinant
Size
100 μg
Price
925.00 EUR
Supplier

Details

Synonyms
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Alternates names
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Taglines
Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Biomolecule/Target
Sonic Hedgehog (SHH)
NCBI Gene ID #.
6469
NCBI Gene Symbol
SHH
Gene Source
Human
Accession #
Q15465
Recombinant
Yes
Source
E. coli
Purity
≥97%
Activity (Specifications/test method)
N/A
Biological activity
Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED₅₀, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
Results
750ng/ml.
Molecular Weight
19.697kDa
Storage Temp.
-20°C
Shipping
Gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
Reconstitution Instructions
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants