Target of this antibody:
Alternative names of antibody target:
KAT5 Antibody: lysine acetyltransferase 5, K-acetyltransferase 5, lysine acetyltransferase 5, HIV-1 Tat interactive protein 60kDa, HTATIP, HTATIP1, TIP, cPLA2 interacting protein, cPLA2, ESA1, PLIP
TIP60 belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and non-histone proteins. TIP60 has been shown to play a role in DNA repair and apoptosis and is thought to play an important role in signal transduction and autophagy. In case of HIV-1 infection, interaction with the viral Tat protein leads to TIP60 polyubiquitination and targets it to degradation. Also, TIP60 contains a chromodomain. TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
TIP60 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human TIP60.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
1 mg/ml in PBS containing 0.02% sodium azide
Affinity chromatography purified via peptide column
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, IF
Western blot: 1 - 2 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/ml.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.