anti-ABCG1 Antibody (NT)
anti-ABCG1 Antibody (NT)
ABCG1 Antibody (NT)
Target of this antibody:
Alternative names of antibody target:
ABCG1; ABC8; WHT1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog
ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 is involved in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.
Clonality of antibody:
This antibody reacts with:
This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
This antibody has been succesfully tested for use in following methods:
Western blot, IHC, IF, FACS
Western blot: ~1:1000, FACS: ~1:10–1:50, IHC: ~1:50-1:100, IF: ~1:10-1:50
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.