anti-His-Tag Antibody (Clone 11E12)
Catalog number
6714-100
Name
anti-His-Tag Antibody (Clone 11E12)
Size
His-Tag Antibody (Clone 11E12)
Price
403.00 EUR
Supplier
Details
Target of this antibody:
His-Tag
Alternative names of antibody target:
His-Tag
Background information:
A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1 κ
Clone:
Clone 11E12
This antibody reacts with:
All
Immunogene sequence:
6X-HIS-KLH
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
1 mg/ml in 0.15 M PBS
Purification method:
Protein A chromatography from in vitro production
This antibody has been succesfully tested for use in following methods:
WB, ELISA
Recommended usage:
ELISA, WB
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation
histidine
French translation
anticorps
