anti-CD-14 Antibody (CT)

Catalog number
6732-100
Name
anti-CD-14 Antibody (CT)
Size
CD-14 Antibody (CT)
Price
316.00 EUR
Supplier

Details

Target of this antibody:
CD-14
Alternative names of antibody target:
CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen=CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form.
Background information:
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the “adapter molecule,” lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
Clone:
N/A
This antibody reacts with:
Human
Immunogene sequence:
This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Purification method:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
This antibody has been succesfully tested for use in following methods:
Western blot, IHC, FACS
Recommended usage:
Western blot: ~1:1000, IHC: ~1:10–1:50, FACS: ~1:10-1:50.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps