Target of this antibody:
Alternative names of antibody target:
MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half-lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastoma. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
Clonality of antibody:
This antibody reacts with:
Human. Predicted reactivity with Mouse, Rat, Bovine and Pig samples.
This MYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from human MYC.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
Supplied in in PBS with 0.09% (W/V) sodium azide.
This antibody is purified through a protein A column, followed by peptide affinity purification.
This antibody has been succesfully tested for use in following methods:
Western blot, FACS.
Western blot: ~1:1000, FC: 1:10 – 1:50.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.