Target of this antibody:
Alternative names of antibody target:
TERT; EST2; TCS1; TRT; Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2
Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat sequences to chromosome ends. In most human somatic cells, telomerase activity is undetectable, and telomeres shorten with successive cell divisions. However, telomerase activity is detectable in immortal cells and in many human tumors. Two candidate mammalian telomerase proteins have been cloned. Human TP1 (for telomerase-associated protein 1), also designated TLP1 in rat (for telomerase protein component 1), is homologous to the Tetrahymena p80 telomerase protein and has been shown to interact with mammalian telomerase RNA. Human TERT (for telomerase reverse transcriptase), also designated hEST2 (for ever shorter telomeres), is homologous to the p123 telomerase protein from Euplotes and to the yeast Est2 protein. Expression of TERT mRNA has been shown to correlate with telomerase activity in various cell lines.
Clonality of antibody:
This antibody reacts with:
This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1104-1132 amino acids from human TERT.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Protein A column, followed by peptide affinity purification.
This antibody has been succesfully tested for use in following methods:
Western blot, IF, IHC, FC.
WB: 1:100 – 1:250.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.