anti-CD73/NT5E Antibody (CT)

Catalog number
anti-CD73/NT5E Antibody (CT)
CD73/NT5E Antibody (CT)
316.00 EUR


Target of this antibody:
Alternative names of antibody target:
NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase
Background information:
CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
Antibody host:
Clonality of antibody:
Rabbit IgG
This antibody reacts with:
Human, Mouse.
Immunogene sequence:
This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
In PBS with 0.09% (W/V) sodium azide.
Purification method:
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
This antibody has been succesfully tested for use in following methods:
Western blot, IHC.
Recommended usage:
WB: 1:1000, IHC: 1:10 – 1:50.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation