Target of this antibody:
Alternative names of antibody target:
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription.
Clonality of antibody:
This antibody reacts with:
KLH-conjugated synthetic peptide of Histone H3 containing acetylated lysine 9 and the phosphorylated serine 10.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
In PBS with 0.05% (W/V) sodium azide.
Whole antiserum from rabbit
This antibody has been succesfully tested for use in following methods:
Western blot, IF, ELISA, ChIP, Dot blot.
IF: 1:500, WB: 1:250, ELISA: 1:1000 – 1:4000, Dot Blot: 1:20000, ChIP: 15 µl/ChIP.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.