anti-Goat Anti-Mouse IgG (H&L) FITC

Catalog number
6919-100
Name
anti-Goat Anti-Mouse IgG (H&L) FITC
Size
Goat Anti-Mouse IgG (H&L) FITC
Price
262.00 EUR
Supplier

Details

Target of this antibody:
Mouse IgG
Alternative names of antibody target:
Anti-mouse IgG, FITC-Linked Antibody, FITC linked secondary antibody
Background information:
Goat Polyclonal Secondary Antibody to mouse IgG (H&L) FITC.
Antibody host:
Goat
Clonality of antibody:
Secondary antibody
Isotype:
Mouse IgG
Clone:
N/A
This antibody reacts with:
Goat
Immunogene sequence:
Mouse IgG (H&L)
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
In 0.01M Sodium phosphate, 0.25 M NaCl, 1% BSA, 50% Glycerol, pH 7.6 and 0.05% Sodium Azide.
Purification method:
Affinity Purified
This antibody has been succesfully tested for use in following methods:
IF, FACS
Recommended usage:
IF: 1:50 – 1:200, FC: 1:50 – 1:200.
Storage information:
Shelf time: 6 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody
Latin name
Capra aegagrus hircus,Mus musculus
Additional isotype
Heavy & Light chains,IgG
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.