Target of this antibody:
Alternative names of antibody target:
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with have either CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells.
Clonality of antibody:
This antibody reacts with:
Human, Mouse, Rat, Dog, Monkey
This CD4 antibody is generated from KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD4. The exact sequence is proprietary.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen Affinity chromatography
This antibody has been succesfully tested for use in following methods:
WB: 1:500 - 1:1000
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.