CCL23/Ck beta 8-1, human recombinant

Catalog number
4276-50
Name
CCL23/Ck beta 8-1, human recombinant
Size
50 μg
Price
588.00 EUR
Supplier

Details

Target
CCL-23
Background
Human Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family. CCL23 is also known as myeloid progenitor inhibitory factor MPIF-1, CK8 and SCYA23. CCL23 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CCL23 (aa 22 -120).Additional N-terminal Processing of the 99 aa residue variant can generate a 75 aa residue peptide (aa 46-120) that is significantly more active than the 99 aa residue variant. CCL23 binds to CCR1 with high affinity and has chemotactic activity for monocytes, dendritic cells, and osteoclast precursors. CCL23 enhances angiogenesis of endothelial cells, but reduces the proliferation of progenitor cells giving rise to granulocyte and monocyte lineages.
Concentration
N/A
Molecular weight
~11.5 kDa
Synonym
G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
Other name
C-C motif chemokine 23, CK-beta-8, CKB-8, Macrophage inflammatory protein 3, MIP-3, Myeloid progenitor inhibitory factor 1, MPIF-1, Small-inducible cytokine A23, CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99)
NCBI gene number
6348
NCBI gene
CCL3
Gene source
Human
NCBI number
P10147
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED50 of rhCCL23 was 0.2-0.5 μg/mL as determined by its ability to chemoattract THP1 human acute monocytic leukemia cells. The ED50 of rhCCL23 was 0.02-0.1 μg/mL as determined by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells.
Reesults
0.2-0.5 μg/ml
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.
How to reconstitute
Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/ml.). This can further be diluted to other aqueous buffers.
Aa sequence
Recombinant Human CCL23 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 100 amino acids with an extra N-terminal Met and having a molecular mass of 11.5 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Val-Thr-Lys.
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants