Target of this antibody:
Alternative names of antibody target:
CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45, IL1BC, IL1BCE, CASP1
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Cleaved-caspase-1 (D210) detects levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to D210.
Clonality of antibody:
This antibody reacts with:
Synthesized peptide derived from human Caspase-1 between Ser 129 - Asn 205
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
50 µl purified antibody supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Affinity purified rabbit IgG
This antibody has been succesfully tested for use in following methods:
Western Blot, Immunohistochemistry, ELISA,
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.