Target of this antibody:
Alternative names of antibody target:
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, GPR49, GPR67
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Clonality of antibody:
This antibody reacts with:
Human, Mouse, Rat
synthetic human LGR5 peptide (Ile685 - Pro720)
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl antibody supplied in PBS with 0.09% (W/V) sodium azide.
Affinity purified rabbit IgG
This antibody has been succesfully tested for use in following methods:
Western Blot, Immunofluorescent staining, Immunohistochemistry, Flow cytometry
Western Blot 1:1000; Immunofluorescent staining 1:100; Immunohistochemistry 1:25; Flow cytometry 1:25
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.