PF 4, human platelets

Catalog number
4356-1000
Name
PF 4, human platelets
Size
1 mg
Price
4889.00 EUR
Supplier

Details

Target
PF 4
Background
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. Human PF-4 a 7.8 kDa protein consisting of 70 amino acid residues.
Concentration
N/A
Molecular weight
7.8 kDa
Synonym
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Other name
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
NCBI gene number
5196
NCBI gene
PLF4
Gene source
Human
NCBI number
P02776
Recombinant
No
Source
Human platelets
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
Binding of HIT-antibodies.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from PBS buffer, pH 7.4.
How to reconstitute
Reconstitute in sterile ddH₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers.
Aa sequence
The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu.
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.