Leptin, human recombinant

Catalog number
4366-5
Name
Leptin, human recombinant
Size
5 mg
Price
640.00 EUR
Supplier

Details

Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.
Alternates names
OB Protein, Obesity Protein, OBS, Obesity factor.
Taglines
Adipose-derived hormone which regulates energy intake & energy expenditure
Biomolecule/Target
Leptin
NCBI Gene ID #.
3952
NCBI Gene Symbol
LEP
Gene Source
Human
Accession #
P41159
Recombinant
Yes
Source
E. coli
Purity
≥98%
Activity (Specifications/test method)
N/A
Biological activity
BioVision’s human Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Results
N/A
Molecular Weight
16.0 kDa
Storage Temp.
-20°C
Shipping
Gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Sterile filtered and lyophilized
Reconstitution Instructions
Add 15 mM sterile HCl (0.5 ml/1 mg vial or 5 ml/10 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1 mg vial or 3 ml/10 mg vial) to bring the pH to approximately 5.2.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
Additional source
Recombinants or rec. proteins
Group
recombinants