Leptin, rat recombinant

Catalog number
4368-5
Name
Leptin, rat recombinant
Size
5 mg
Price
665.00 EUR
Supplier

Details

Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.
Alternates names
OB Protein, Obesity Protein, OBS, Obesity factor.
Taglines
Adipose-derived hormone which regulates energy intake & energy expenditure
Biomolecule/Target
Leptin
NCBI Gene ID #.
25608
NCBI Gene Symbol
LEP
Gene Source
Rat
Accession #
P50596
Recombinant
Yes
Source
E. coli
Purity
≥95%
Activity (Specifications/test method)
N/A
Biological activity
The ED₅₀ determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.
Results
0.35-0.06 ng/ml
Molecular Weight
16.2 kDa
Storage Temp.
-20°C
Shipping
Gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Lyophilized from 4.5 µM NaHCO₃.
Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Additional source
Recombinants or rec. proteins
Group
recombinants