anti-Anti-phospho-Rsk1 (Thr359/Ser363), Rabbit Monoclonal Antibody

RPS6KA1

rsk-1, rsk 1, Active RSK1, RSK1, RSK, human RSK1, active kinase, active kinases, recombinant RSK1, recombinant RSK, kinase activity assay, protein kinase

The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90(rsk). RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. Moller described the cloning and characterization of 3 genes encoding RSKs, and HU1 (also named RPS6KA1, or RSK1) cDNA encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. Northern blot and RNase protection analyses detected an approximately 3.5-kb HU1 transcript in lymphocytes, skeletal muscle, liver, and adipose tissue. Zeniou determined the expression of the RSK1, RSK2, and RSK3 genes in various human tissues, during mouse embryogenesis, and in mouse brain. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation.

Rabbit

Monoclonal

Rabbit IgG

RM233

Human

A phospho-peptide corresponding to human phospho-Rsk1 (Thr359/Ser363)

Liquid

In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Affinity purified using protein A

IHC, WB

WB: 1:1000 - 1:2000 dilution; IHC: 1:200 -1:500 dilution

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Oryctolagus cuniculus

anticorps