anti-RASSF1A Antibody

RASSF1

Ras association domain-containing protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21

RASSF1A is one of the most epigenetically silenced gene in human cancers and an important negative regulator of NFkB activation and potential tumor suppressor. It is required for death receptor-dependent apoptosis. It also mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. RASSF1A inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles; no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

Mouse

Monoclonal

Mouse IgM ĸ

C10

Human, Mouse

Full length protein

Liquid

In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide

Affinity purified

WB, IH, IP

WB- 1:50, IHC- 1:50, IP- 1.5 µg

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps