Leptin Receptor, human recombinant

Catalog number
4582-50
Name
Leptin Receptor, human recombinant
Size
50 μg
Price
745.00 EUR
Supplier

Details

Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor
Alternates names
OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295
Taglines
A receptor for the fat cell-specific hormone leptin
Biomolecule/Target
Leptin Receptor
NCBI Gene ID #.
3952
NCBI Gene Symbol
LEP
Gene Source
Human
Accession #
P41159
Recombinant
Yes
Source
Human
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032 - 4.0 μg/ml.
Results
N/A
Molecular Weight
95.0 kDa (predicted) 130-140 kDa on a gel.
Storage Temp.
-70°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. The lyophilized Leptin Binding Domain is very soluble in H₂O and most aqueous buffers below and above the isoelectric point (~ 5.9).
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Additional source
Recombinants or rec. proteins
Group
recombinants