Follistatin, human recombinant

Catalog number
4708-20
Name
Follistatin, human recombinant
Size
20 μg
Price
246.00 EUR
Supplier

Details

Background
Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-β ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-β1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.
Concentration
N/A
Molecular weight
31.5 kDa
Synonym
FST, FS, Activin-binding protein
Other name
FST, FS, Activin-binding protein
NCBI gene number
10468
NCBI gene
FST
Gene source
Human
NCBI number
P19883
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED₅₀ is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.
Reesults
0.1-0.4 µg/ml
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl
How to reconstitute
Reconstitute in H₂O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants