Human CellExp™ LEFTY-I, Human Recombinant

Catalog number
4873-50
Name
Human CellExp™ LEFTY-I, Human Recombinant
Size
50 μg
Price
1409.00 EUR
Supplier

Details

Background
BioVision’s LEFTY-1 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 38 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. Lefty proteins function as an antagonist of the Nodal Signaling pathway. Proper functioning of Lefty is crucial to the proper development of the heart, lungs, spleen, and liver.
Concentration
N/A
Molecular weight
38 kDa
Synonym
Human Recombinant LEFTY-I
Other name
Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B
NCBI gene number
10637
NCBI gene
LEFTY1
Gene source
Human
NCBI number
O75610
Recombinant
Yes
Source
Human 293 cells
Purification
≥90%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse Chondrogenic cell line).
Reesults
Typically 10 to 40 ng/mL ED50.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Blue ice
Physical appearance
Lyophilized protein
Physical properties
Lyophilized in 10 mM Tris-HCl, pH 7.4, 50 mM NaCl, 0.5% CHAPS.
How to reconstitute
Reconstitute in sterile water.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants