Human CellExp™ Cystatin C, Human Recombinant

Catalog number
4878-50
Name
Human CellExp™ Cystatin C, Human Recombinant
Size
50 μg
Price
806.00 EUR
Supplier

Details

Background
Xeno-free Cystatin C HuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
Concentration
N/A
Molecular weight
12-13 kDa
Synonym
Human Recombinant Cystatin C
Other name
Cystatin-3, Gamma-trace neuroendocrine basic polypeptide, Post-gamma-globulin
NCBI gene number
1472
NCBI gene
CST4
Gene source
Human
NCBI number
P01036
Recombinant
Yes
Source
Human 293 cells
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 µg/mL (0.3 to 1.5 µM) with a range of 1.56 µg/mL to 50 µg/mL Cystatin C in presence of 0.55 µM papain and 0.44 µM L-BAPA.
Reesults
Typically 0.3 to 1.5 µM IC50.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Blue ice
Physical appearance
Lyophilized protein
Physical properties
Lyophilized in PBS
How to reconstitute
Reconstitute in sterile PBS.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants