Caspase-3 Substrate DEVD-pNA for cell activation

Catalog number
1008-1000
Name
Caspase-3 Substrate DEVD-pNA for cell activation
Size
1000 assays
Price
643.00 EUR
Supplier

Details

Description
Ready-to-use colorimetric substrate for CPP32/caspase-3 and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate DEVD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amounts of caspase assays.
Peptide sequence
Ac-Asp-Glu-Val-Asp-pNA
CAS number
N/A
Molecular weight
638.58
Category
Caspase Substrate
Other name
Ac-DEVD-pNA, Caspase-3 Substrate, Colorimetric
Molecular formula
C₂₆H₃₄N₆O₁₃
Physical appearance
Liquid
Supplied with
In DMSO ( 4 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>98% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
6-12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.