Caspase-8 Substrate IETD-AFC for cell activation

Catalog number
1062-1000
Name
Caspase-8 Substrate IETD-AFC for cell activation
Size
1000 assays
Price
643.00 EUR
Supplier

Details

Description
Ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Peptide sequence
Ac-Ile-Glu-Thr-Asp-AFC
CAS number
N/A
Molecular weight
729.7
Category
Caspase Substrate
Other name
Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic
Molecular formula
C₃₁H₃₈F₃N₅O₁₂
Physical appearance
Liquid
Supplied with
DMSO (2 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>95% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.