Caspase-2 Substrate VDVAD-AFC for cell activation

Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence VDVAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate VDVAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.

Ac-Val-Asp-Val-Ala-Asp-AFC

N/A

770.7

Caspase Substrate

Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic, VDVAD-AFC, caspase2 substrate, caspase 2 substrate

C₃₃H₄₁F₃N₆O₁₂

Liquid

DMSO ( 1 mM)

No

No

>98% by HPLC

N/A

-20°C

gel pack

12 months

Protect from light and moisture

cell

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.