Caspase-9 Substrate LEHD-AFC for cell activation

Catalog number
1075-1000
Name
Caspase-9 Substrate LEHD-AFC for cell activation
Size
1000 assays
Price
643.00 EUR
Supplier

Details

Description
Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.
Peptide sequence
Ac-Leu-Glu-His-Asp-AFC
CAS number
N/A
Molecular weight
765.7
Category
Caspase Substrate
Other name
Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic
Molecular formula
C₃₃H₃₈F₃N₇O₁₁
Physical appearance
Liquid
Supplied with
DMSO ( 1 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>98% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.