Caspase-1 Substrate YVAD-AFC for cell activation

Catalog number
1103-200
Name
Caspase-1 Substrate YVAD-AFC for cell activation
Size
200 assays
Price
327.00 EUR
Supplier

Details

Description
Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.
Peptide sequence
Ac-Tyr-Val-Ala-Asp-AFC
CAS number
N/A
Molecular weight
719.7
Category
Caspase Substrate
Other name
Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
Molecular formula
C₃₃H₃₆F₃N₅O₁₀
Physical appearance
Liquid
Supplied with
DMSO ( 1 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>99% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.