PARP-1, human recombinant

Catalog number
4992-50
Name
PARP-1, human recombinant
Size
50 μg
Price
495.00 EUR
Supplier

Details

Synonyms
Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP
Alternates names
Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP
Taglines
Involved in DNA repair and programmed cell death
Biomolecule/Target
NA
NCBI Gene ID #.
142
NCBI Gene Symbol
PARP-1
Gene Source
Human
Accession #
P09874
Recombinant
Yes
Source
Baculovirus
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
N/A
Biological activity
1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 μg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.
Results
N/A
Molecular Weight
116.0 kDa
Storage Temp.
-70°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Lyophilized solid
Reconstitution Instructions
Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 μL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 μL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants