G418 Sulfate for cell activation

Catalog number
1557-1G
Name
G418 Sulfate for cell activation
Size
1 g
Price
137.00 EUR
Supplier

Details

Description
Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
Peptide sequence
N/A
CAS number
108321-42-2
Molecular weight
692.71
Category
Biochemicals
Other name
Antibiotic G418, G418
Molecular formula
C₂₀H₄₀N₄O₁₀. 2 H₂SO₄
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
Yes
Is it cell-permeable?
No
Purification
>720 U/mg (potency)
Reconstitute instructions
H₂O (50 mg/ml)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
24months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.