Human recombinant UBE2D2 (UbcH5b)

Catalog number
6435-3
Name
Human recombinant UBE2D2 (UbcH5b)
Size
3 nmol
Price
365.00 EUR
Supplier

Details

Background
UBE2D2 (UbcH5b) is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. The UbcH5 family (including UbcH5a and UbcH5c, 88% and 89% sequence identity with UbcH5b, respectively) are the human homologues of the Saccharomyces cerevisiae UBC4/5 family (~79% sequence identity). Members of the UbcH5 family have been implicated in a number of inflammatory and cancer pathways, including the ubiquitylation of p53 (involving the E3 ligase Hdm2) and IκBα (involving the E3 ligase SCF).
Concentration
N/A
Molecular weight
17.729 kDa with His Tag.
Synonym
Human recombinant UBE2D2 (UbcH5b)
Other name
Human recombinant UBE2D2 (UbcH5b), ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC4, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, UBCH5B, EC 6.3.2.19, E2(17)KB2, PUBC1, UBC4/5, UBC5B, UBCH4.
NCBI gene number
7322
NCBI gene
UBE2D2
Gene source
Human
NCBI number
P62837
Recombinant
Yes
Source
N/A
Purification
≥95%
Tested applications
RP-HPLC, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants