Human recombinant UBE2E3 (UbcH9)

Catalog number
6439-3
Name
Human recombinant UBE2E3 (UbcH9)
Size
3 nmol
Price
365.00 EUR
Supplier

Details

Background
Ubiquitin-conjugating enzyme E2 E3 (UBE2E3 alias UbcH9) is a protein that in humans is encoded by the UBE2E3 gene. The deduced 207-amino acid protein shares over 94% amino acid identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the N-terminal extension exhibited little homology among these, except for UbcM2, which showed 100% identity, and which is thought to be a mouse counterpart. The UBE2E3 form a thioester bond with ubiquitin (Ub) in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.
Concentration
N/A
Molecular weight
22.913 kDa
Synonym
Human recombinant UBE2E3 (UbcH9)
Other name
Ubiquitin-conjugating enzyme E2E 3, Ubiquitin-conjugating enzyme E2-23 kDa, UBCH9, Ubiquitin-protein ligase E3, Ubiquitin-conjugating enzyme E2E3.
NCBI gene number
10477
NCBI gene
UBE2E3
Gene source
Human
NCBI number
Q969T4
Recombinant
Yes
Source
N/A
Purification
≥95%
Tested applications
RP-HPLC, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants