Human recombinant UBE2H (UbcH2), HIS6SUMO

Catalog number
6441-3
Name
Human recombinant UBE2H (UbcH2), HIS6SUMO
Size
3 nmol
Price
393.00 EUR
Supplier

Details

Background
Ubiquitin-conjugating enzyme E2 H (UBE2H) is a member of the ubiquitin-conjugating enzyme family. Protein modification with ubiquitin is a vital cellular apparatus for directing abnormal or short-lived proteins for degradation. Ubiquitination requires at least 3 classes of enzymes: ubiquitin-activating enzymes (E1s) ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2H protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and Zebrafish homologs.
Concentration
N/A
Molecular weight
32.675 kDa. (His6-SUMO Tagged)
Synonym
Human recombinant UBE2H (UbcH2), HIS6SUMO
Other name
Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H, GID3, UBC8, UBCH.
NCBI gene number
7328
NCBI gene
UBE2H
Gene source
Human
NCBI number
P62256
Recombinant
Yes
Source
N/A
Purification
≥90%
Tested applications
RP-HPLC, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants