Human CellExp™ Beta-NGF, Human Recombinant

Catalog number
6443-10
Name
Human CellExp™ Beta-NGF, Human Recombinant
Size
10 μg
Price
284.00 EUR
Supplier

Details

Background
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. The presence of beta NGF in immune cells and endocrine cells as well as in the CNS limbic areas suggests that beta NGF may function as an intracellular messenger to regulate the body’s response to stress. This product is produced in a human cell expression system with serum-free, chemically defined media.
Concentration
N/A
Molecular weight
13 kDa, non-disulfide bonded homodimer, non-glycosylated.
Synonym
Human Cellexp Human Recombinant Beta-NGF
Other name
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428
NCBI gene number
4803
NCBI gene
NGF
Gene source
Human
NCBI number
P01138
Recombinant
Yes
Source
Human cell expressed
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 1.5 to 8 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Reesults
1.5 to 8 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from a PBS solution.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants