Human CellExp™ FGF-2/FGF-basic, Human Recombinant

Catalog number
6448-50
Name
Human CellExp™ FGF-2/FGF-basic, Human Recombinant
Size
50 μg
Price
648.00 EUR
Supplier

Details

Background
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Concentration
N/A
Molecular weight
17 kDa, monomer, non-glycosylated
Synonym
Human Cellexp Human Recombinant FGF-basic
Other name
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
NCBI gene number
2247
NCBI gene
FGF2
Gene source
Human
NCBI number
P09038
Recombinant
Yes
Source
Human cell expressed
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line.
Reesults
0.1 to 0.5 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from a PBS solution.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants